Bio-One of Flagstaff decontamination and biohazard cleaning services

For the 3rd Time, Bio-One Appeared on the Inc. 5000, Ranking No. 4,365

Denver, CO August 19, 2022 – Inc. revealed that Bio-One is No. 4,365 on its annual Inc. 5000 list, the most prestigious ranking of the fastest-growing private companies in America. The list represents a one-of-a-kind look at the most successful companies within the economy’s most dynamic segment—its independent businesses. Facebook, Chobani, Under Armour, Microsoft, Patagonia, and many other well-known names gained their first national exposure as honorees on the Inc. 5000. 
Sandi Ellis, President of Bio-One, had this to say about the achievement: “We are honored to be recognized and this is only possible because of our amazing Bio-One owners across the United States. Every Bio-One owner works hard in their community, and we thank them for their time, dedication, and sacrifices.”
The companies on the 2022 Inc. 5000 have not only been successful, but have also demonstrated resilience amid supply chain woes, labor shortages, and the ongoing impact of Covid-19. Among the top 500, the average median three-year revenue growth rate soared to 2,144 percent. Together, those companies added more than 68,394 jobs over the past three years. 
Complete results of the Inc. 5000, including company profiles and an interactive database that can be sorted by industry, region, and other criteria, can be found at The top 500 companies are featured in the September issue of Inc. magazine, which will be available on August 23. 
“The accomplishment of building one of the fastest-growing companies in the U.S., in light of recent economic roadblocks, cannot be overstated,” said Scott Omelianuk, editor-in-chief of Inc. “Inc. is thrilled to honor the companies that have established themselves through innovation, hard work, and rising to the challenges of today.” 
Ellis said, “While the ranking is a wonderful reflection of the company’s growth in the past 12 years, I am also excited for the future of Bio-One. We’re investing in new technology, improving our digital strategies, and are in the middle of launching a new brand. It’s an exciting time to be part of Bio-One!”
Bio-One Inc. is the first crime and trauma scene cleaning franchise with over 125 locations. Each location is owned and operated locally by caring members of their respective communities. In an effort to boost business growth and impact more areas, Bio-One was acquired by Five Star Franchising last year. Bio-One has been ranked in Entrepreneur magazine’s 43rd Annual Franchise 500 for 2022, and in 2021 placed number one in Entrepreneur’s Best of the Best for Crime Scene Cleaning and Trauma Scene Cleaning.
Sandi Ellis
(303) 625-6543

More about Inc. and the Inc. 5000 

Companies on the 2022 Inc. 5000 are ranked according to percentage revenue growth from 2018 to 2021. To qualify, companies must have been founded and generating revenue by March 31, 2018. They must be U.S.-based, privately held, for-profit, and independent—not subsidiaries or divisions of other companies—as of December 31, 2021. (Since then, some on the list may have gone public or been acquired.) The minimum revenue required for 2018 is $100,000; the minimum for 2021 is $2 million. As always, Inc. reserves the right to decline applicants for subjective reasons. Growth rates used to determine company rankings were calculated to four decimal places. The top 500 companies on the Inc. 5000 are featured in Inc. magazine’s September issue. The entire Inc. 5000 can be found at
About Inc. 
The world’s most trusted business-media brand, Inc. offers entrepreneurs the knowledge, tools, connections, and community to build great companies. Its award-winning multiplatform content reaches more than 50 million people each month across a variety of channels including websites, newsletters, social media, podcasts, and print. Its prestigious Inc. 5000 list, produced every year since 1982, analyzes company data to recognize the fastest-growing privately held businesses in the United States. The global recognition that comes with inclusion in the 5000 gives the founders of the best businesses an opportunity to engage with an exclusive community of their peers, and the credibility that helps them drive sales and recruit talent. The associated Inc. 5000 Conference & Gala is part of a highly acclaimed portfolio of bespoke events produced by Inc. For more information, visit
For more information on the Inc. 5000 Conference & Gala, visit

First Responder Relay Presented by Bio-One, Inc.

We are delighted to announce our sponsorship of the First Responder Relay in Las Vegas on October 16th. The First Responders Relay is a 9 person 48-mile running relay with stages ranging from 4.0 miles to 7.7 miles. The relay is competition comprised of first responder personnel from throughout the United States and the World, and is hosted by the California Police Athletic Federation (CPAF).

There is still time to register, but don't delay! Registration closes September 18.  Visit to join us in Las Vegas!

Bio-One is the Presenting Sponsor at the 2021 United State Police and Fire Championships is Proud To Show Some Pictures From the Event



Bio-One has been honored to be the presenting sponsor for the 2021 USPFC. Athletes from all over the county have come to San Diego, Ca to compete in competitions from everything from surfing to CrossFit, cornhole to soccer and everything in between. The athletes are all police, firefighters, FBI, corrections, border patrol and other departments. And the athletes are either current first responders or are retired. The championships are being held June 10-19th all over the San Diego area so we are right in the middle of the competition. Once all of the events are finished, we will be posting an update. But until then, please enjoy these photos we have from the past few days of the event. Please contact us with any questions you may have. 928-863-8276 or
















National Police Week Badge


Bio-One Donates First Aid Kits in Recognition of National Police Week


Flagstaff, AZ (May 12, 2021) – In recognition of National Police Week, May 9-15, Bio-One thanked first responders by donating first aid kits to Holbrook Police Department, NAU Police Department, Sedona Police Department and Prescott Valley Police Department as part of the nationwide campaign #HelpFirst for First Responders. Throughout the week, Bio-One offices in 41 states distributed a cumulative of 5,000 first aid kits, which included alcohol swabs, Advil, gloves, gauze pads, and an assortment of bandages.


The #HelpFirst campaign aligns with the Bio-One motto, Help First, Business Second, and will be used to help officers and community members in the event of a minor medical emergency.


“Bio-One always has and always will support our local law enforcement” said Rebecca Wallace, owner of Bio-One. “This campaign is just a small way of letting our local LEOs know that we see them and appreciate them.”


As a 2021 corporate sponsor of the Law Enforcement Officers Memorial Fund, Bio-One, Inc. will also pay tribute to fallen officers during the virtual 33rd Annual Candlelight Vigil on May 13 at 8:00 p.m. The event will be broadcasted live on Facebook, YouTube, and


“We are so thankful for Bio-One’s corporate partnership and their continued support of the Memorial Fund,” said Marcia Ferranto, CEO of the National Law Enforcement Memorial Fund. “This partnership will further bolster the Memorial Fund’s mission of honoring America’s Law Enforcement, as well as provide support for the Museum’s upcoming exhibition opening in September. We are grateful for their commitment.” 


Established in 1984, the National Law Enforcement Officers Memorial Fund is a nonprofit organization dedicated to honoring the fallen, telling the story of American law enforcement, and making it safer for those who serve. The Memorial Fund maintains the National Law Enforcement Officers Memorial in Washington, D.C., which honors the names of all of the 22,611 officers who have died in the line of duty throughout U.S. history. 


About Bio-One 

Bio-One, a crime and trauma scene cleaning franchise, operates in Flagstaff, Sedona, Prescott Valley, Prescott, Holbrook, Show Low, Pinetop-Lakeside, Snowflake, Page and all other parts of Northern Arizona. Bio-One is committed to providing excellent service in recovery and suicide cleanup, homicide cleanup, hoarding situations, junk removal, deceased animal recovery, feces removal, pest and rodent droppings and much more. Each Bio-One office is independently owned and operated by a member of the local community. For more information about Bio-One, visit us at  




The California Police Athletic Federation (CPAF) is proud to announce that the 2021 United States Police & Fire Championships (USPFC) are now Presented by Bio-One, Inc. 


Operating under the motto, "Help First, Business Second," Bio-One provides high-level decontamination and biohazard cleanup services while offering clients the privacy and compassion needed at difficult times. Bio-One operates in 41 states with over 110 locations and is committed to providing first-class service. 


"We are thrilled to add Bio-One as the Presenting Sponsor for the 2021 USPFC because   First Responders warrant our support," said California Police Athletic Federation President LC Collins. "Bio-One’s reputation is second to none, and with their partnership, we can offer first responders a health and wellness outlet.  We look forward to working with them for years to come."


Founded by San Diego Police Lieutenant Veon "Duke" Nyhus, The United States Police & Fire Championships were first held in San Diego in 1967. Duke recognized the need to promote physical fitness and camaraderie among the Public Safety and First Responder community members. 


The USPFC caters to active and retired public safety and first responders who participate in Olympic-style competitions and open to individuals representing firefighters, law enforcement, and officers from corrections, probation, border protection, immigration, and customs across the country. Traditionally athletes compete in 45+ sporting events from biathlon to motocross across 35+ venues in Southern California.


"At Bio-One our support for Law Enforcement and Fire Service Officers is unwavering, and we're thrilled to partner with the California Police Athletic Federation during this memorable event," said Nick-Anthony Zamucen, founder of Bio-One. "We wish each athlete success and look forward to celebrating these everyday heroes."


The 2021 USPFC Presented by Bio-One will take place from June 10th to the 19th in venues across San Diego, California, and strengthen the relationship between First Responders and the community for many years to come!


About the United States Police and Fire Championships

The California Police Athletic Federation (CPAF) is the parent organization of two multi-sport programs designed for peace officers and firefighters. The World Police & Fire Games (WPFG) are open to active and retired law enforcement and fire service personnel throughout the world. The United States Police & Fire Championships (USPFC) are open to active or retired public safety and first responder personnel from an eligible agency within the USA. For more information, please visit 


National Telecommunications Week

The second week of April is a time to celebrate and thank telecommunications professionals who dedicate their lives to serving the public. Often called the thin gold line, 911 dispatchers are true heroes behind the scenes. 


"This week-long event, initially set up in 1981 by Patricia Anderson of the Contra Costa County Sheriff’s Office in California, is a time to celebrate and thank those who dedicate their lives to serving the public. It is a week that should be set aside so everyone can be made aware of their hard work and dedication." - Association of Public-Safety Communications Officials (APCO) International


Here, in Northern Arizona our Bio-One team would like to say "Thank You" to the 911 dispatchers that are critical to our community. 




Bio-One ranked number one in Entrepreneur magazine’s 42nd Annual Franchise 500 for success in crime-scene and trauma-scene cleaning. This marks the fifth year in a row that Bio-One has been included in the ranking.

Flagstaff, AZ Bio-One ranked number one in Entrepreneur magazine’s 42nd Annual Franchise 500 for success in crime-scene and trauma-scene cleaning. This marks the fifth year in a row that Bio-One has been included in the ranking.

Bio-One is locally owned and operated by Joshua and Rebecca Wallace. Joshua and Rebecca Wallace have been serving the Flagstaff and Northern Arizona community since 2018.


Bio-One provides service in recovery and suicide cleanup, homicide cleanup, hoarding situations, junk removal, deceased animal recovery, feces removal, pest and rodent droppings and more.


Owner Rebecca Wallace recently wrote an article and said, “When looking at purchasing a Bio-One office, the first thing that stuck out to us wasn’t the fact that we would be cleaning what is impacted after a suicide, homicide, unattended death or so-on. What stuck out to us was “Help First, Business Second.” We wanted to help people and make a difference in our community.

We get to help people during some of the hardest times in their lives. Normally, we are the last people they see right after tragedy. The police, EMTs and medical examiners have left, and then we come in. Because of that, we also make sure to have all of the resources the family may need. We have a responsibility to take care of people when they need us and help them however we can. It is why we do what we do.”


The number of Bio-One franchises have increased 145 percent in the last three years with over 100 locations in 41 states. “We’re honored to be recognized by Entrepreneur,” said Nick-Anthony Zamucen, CEO of Bio-One Inc. “The ranking is a direct reflection of our Bio-One family, strength of our business model, and our ability to consistently deliver care and compassion to our clients.”


Over its 42 years in existence, the Franchise 500® has become both a dominant competitive measure for franchisors and a primary research tool for potential franchisees. Bio-One’s position on the ranking is a testament to its strength as a franchise opportunity.



About Bio-One Inc.
Bio-One Inc. is the first crime and trauma scene cleaning franchise, operating in 41 states with over 100 locations. Bio-One is committed to providing excellent service in recovery and suicide cleanup, homicide cleanup, hoarding situations, junk removal, deceased animal recovery, feces removal, pest and rodent droppings and much more. Each office is independently owned and operated by a member of the local community. For more information about Bio-One, visit us at




Bio-One Gives Back 2020

Every year we look for ways to give back to our community and help those in need. Bio-One lives by the moto, "Help First, Business Second" every chance we get. 2020 has proven to be especially trying for all of us, so giving back has been needed now more than ever. We racked our brains on how this would look. As a company who primarily helps people after the tragedy of suicide, homicide, hoarding, undiscovered deaths and infectious disease decontamination, what could we do? What we decided, is that we would provide as many first responders and essential businesses as possible with chemicals approved by the EPA for COVID-19 decontamination. So from March-December 2020 we handed out over 20,000 bottles of chemicals to police, fire, EMS, apartments, animal rescues, victim advocates, family advocacy centers and so many more. We saw a need and answered it as best we could. 



Another thing we did this year was provide over 200 crisis responders and victim advocates with holiday goodies. These men and women have not stopped working during the pandemic. They come into homes after a homicide, suicide, rape, domestic violence, house fire and anything in between. They give the victims or surviving family the resources and support they need to move forward and navigate the next steps. These advocates and crisis responders are so important and vital, yet many do not know about them. So we wanted to show how much we appreciate them. Victim advocates are in almost every single city in the US. If you think you need an advocate for a situation you are dealing with, please reach out to them. 



As a community, we need to all step up to help one another. This year has been extremely rough and so many are in very bad places. If you are able to help, even if just to donate some of your time, there are many organizations who can use the help. Check with your local animal shelters, food banks, Boys and Girls Club, churches and senior living facilities. The way you can help has changed due to COVID-19, but you can still help


For more information on what you can do in your community please call or email us 928-863-8276  480-276-4061


Animal Hoarding
The Ugly Truth

OCT 08, 2020 by Rebecca Wallace  


Animal hoarding is something that is more common than you might realize. Sometimes you will hear about it in the news, magazines and social media. You will hear how the animals were rescued and the person who is responsible for their mistreatment has charges against them. But what happens to the animals? Who rescues them and what do they go through? What happens to the home? 

There are many aspects to take into consideration when talking about animal hoarding. One of the biggest is who is going to take the animals and what condition are the animals in. When there are large numbers of animals who need to be removed from a home a one time, most times, multiple rescues have to help. As much as rescues prepare for any situations, you can never really be prepared for 100+ animals, who are probably sick, to come into your facility all at once. Recently there was a cat hoarding situation in Peoria, Arizona in which 133 cats were being kept in an apartment. Missy from La Gattara Cat Cafe was the first person contacted to help. The cats ranged from newborn kittens, pregnant cats and adult cats and some were sick and most were not spayed or neutered. Also, as you can imagine the living conditions were unimaginable and the air quality was so bad you could barely breath. Missy knew she could not take all of the cats by herself, she needed help. Missy put out the call for help to other rescues across Arizona and many answered the call. 23 of the cats went to a rescue, Ark Cat Sanctuary in Flagstaff, Arizona. Ark Cat Sanctuary gets so many calls for hoarding, that when Missy called, they not only took 23 of the cats, but they put a plan in place to build a habitat specifically for cats coming out of hoarding situations. What a lot of people do not understand is when animals are in such bad living conditions coming out of that they quickly need to be assessed for disease, parasites and trauma. They cannot immediately go into a new home. They need to be rehabilitated and that takes time and money. The rescue community relies on fundraising, grants and volunteers to help. 


Once the animals are rescued from the home, there is a lot of hard work that needs to be done to make the home habitable again. There are many companies that do clean out homes from hoarding situations. However, there is 1 company that is preferred, and that is Bio-One. Bio-One works hand in hand with rescues to make sure all animals are out safely. When there are very young puppies, kittens or elderly animals, they like to hide. So it takes care and focus to look for these animals and get them out of the home into the rescues arms safely. Also, Bio-One always has a staff member on site that is trained in animal rescue and pet first aid in case an emergency situation occurs. While this is taking place, Bio-One is also removing debris, feces, urine, damaged items and anything else that needs to go. Many times there is thousands of dollars in damage in the home. Making sure you have a company that cares is very important because these situations are very sensitive. 


The last piece of the equation is mental health. Hoarding is recognized as a disease and should be treated as so. People who hoard need physiological help or they will most likely revert back to hoarding. Do not turn your back on them, offer them help. The other people who may need a therapist to talk to are the rescuers. They see a part of humanity that many do not want to acknowledge is there or do not understand. They see abuse, death and trauma daily. Yet, they do it day after day. The psychological tole that takes is huge. 


So what can you do to help? 

First, if you are looking for a new pet, please consider adopting from a rescue. It is so rewarding and helps save a life.

Second, if you are looking to volunteer, please call your local animal rescue to volunteer with them. Most rescues are looking for fosters, adoption specialists, comfort volunteers to sit or play with the animals, and many other things.  

Third, donate to your local rescue. Blankets, food, litter and toys are common things you can easily buy that a rescue can use. 

Fourth, if you know someone is a hoarder, please say something to them gently and with love. Offer them support. 


If you have any questions you can reach out to us at any time. We are always here to help. | 928-863-8276 | | 480-276-4061


Local and National Resource Guide
When You Feel Alone, There is Always Someone to Listen



If you or someone you know is having suicidal thoughts or in crisis, there are many different resources available that can help. This can include depression, substance abuse, physical abuse, suicidal thoughts and behavior. Here are a few phone numbers to remember. They could save your life.  


National Hotlines

National Suicide Prevention Helpline – available 24/7 – call (800) 273-8255

National Suicide Prevention Helpline (hearing impaired) – call (800)-799-4889

The Samaritans – available 24/7 and offers emotional support and resources – call or text (877) 870-4673

National Substance Use and Disorder Hotline – available 24/7 for treatment and referrals – (800)662-4357

Crisis Text Line – available 24/7 when in crisis – text “Home” to 741741


Teen and Youth Hotlines

Trevor Project Lifeline – available 24/7 for youth in the LGBTQ community – call (866) 488-7386

Child Help USA National Hotline – available 24/7 for children who are suffering from child abuse – call (800) 422-4453

Boys Town National Hotline – available 24/7 for children and families – call (800) 448-3000

National Teen Dating Violence Hotline – available 24/7 for teens having concerns with a relationship – call (866) 331-9474 or text “loveis” to 22522


Veterans Services

Veterans Crisis Line – available 24/7 – (800) 273-8255 (press 1)

Be Connected – available 24/7 – (866) 4AZ-VETS (429-8387)



Local Arizona Hotlines

Crisis Response Network – available 24/7

            Central Arizona – (602)222-9444

            Central Arizona – (800)631-1314

            Central Arizona (hearing impaired) – (800)327-9254

            Northern Arizona – (877)756-4090

Mercy Care – available 24/7 in Maricopa County – call (800)631-1314 or (602) 222-9444

Arizona Complete Health – available 24/7 in Cochise, Graham, Greenlee, La Paz, Pima, Pinal, Santa Cruz and Yuma Counties served – call (866) 495-6735

Health Choice Arizona – available 24/7 in Apache, Coconino, Gila, Mohave, Navajo and Yavapai Counties – call (877) 756-4090

Gila River and Ak-Chin Indian Communities – call (800)-259-3449


Salt River Pima Maricopa Indian Community – call (855) 331-6432


Making the phone call can be one of the hardest things to do, but it can save your life. There is always someone to listen to you and give you resources. You are not alone.


For more resources or if you have any questions, feel free to call Bio-One at 480-276-4061 or 928-863-8276. You can also reach us by email at or



Photo by Simon Migaj from Pexels




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Bio-One of Flagstaff

Bio-One of Flagstaff decontamination and biohazard cleaning services

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